Quantabio UltraPlex 1-Step ToughMix (4X) L-ROX, 1000R 95168-01K

UltraPlex 1-Step ToughMix (4X) L-ROX is a ready-to-use, 4X concentrated master mix for one-step reverse transcription and real-time quantitative PCR (RT-qPCR) of RNA templates using probe-based detection. The formulation includes reverse transcriptase, RNase inhibitor and hot-start Taq DNA polymerase with optimized buffers and stabilizers, and is supplied with Low ROX passive reference dye. It is designed for sensitive, multiplex RT-qPCR workflows and for applications where template-derived PCR inhibitors may be present, enabling convenient ambient-temperature reaction setup and probe-based quantification of up to five targets in a single reaction.

Key Features:

• Concentration: 4X concentrated RT-qPCR master mix
• Passive reference dye: Low ROX (L-ROX)
• Pack size: 1000 reactions (1000 x 20 µL rxns; supplied as 1 x 5 mL)
• Manufacturer part code: 95168-01K
• Multiplex capability: up to 5 targets per reaction
• Includes qScript XLT reverse transcriptase, RNase inhibitor, and AccuStart II hot-start Taq polymerase

Advantages:

• High sensitivity for low-copy RNA detection enabled by a concentrated, optimized enzyme mix
• Multiplexing up to five targets reduces sample consumption and increases throughput
• Enhanced tolerance to PCR inhibitors supports use with crude extracts, clinical specimens, and environmental samples
• Temperature-stabilized formulation permits convenient reaction setup at ambient room temperature