Quantabio sparQ Universal Library Quant Kit (100rxn) 95210-100

sparQ Universal Library Quant Kit is a qPCR-based kit for rapid and accurate quantification of libraries prepared for sequencing on Illumina NGS platforms. The kit measures the number of amplifiable library molecules that possess the appropriate adapter tags at both ends, enabling optimal cluster generation and efficient use of sequencing capacity. The 100 rxn format supplies ready-to-use reagents and protocols for complete library quantification workflows with fast cycling compatible with common qPCR instruments.

Key Features:

• Format: 100 rxn
• Application: qPCR quantification of Illumina NGS libraries
• Time to result: complete quantification in approximately 40 minutes (≈50% shorter run time vs traditional cycling protocols)
• Reaction volumes: optimized for 10 µL or 20 µL reaction setups
• Contains stabilized, ready-to-use sparQ Universal Fast Mastermix to reduce pipetting steps
• Compatible with a wide range of qPCR instruments and dyes (No ROX / Low ROX / High ROX workflows supported)

Advantages:

• Faster time-to-results—reduces qPCR run time by approximately 50% for quicker library QC
• Accurate and reliable quantification across varied library sizes and GC contents to support optimal flow cell loading
• Minimized pipetting and simplified setup through a stabilized, ready-to-use mastermix
• High amplification efficiency and superior run-to-run uniformity for precise and reproducible measurements