Quantabio sparQ RNA-Seq HMR Kit, 96 rxns 95216-096

Quantabio sparQ RNA-Seq HMR Kit is a 96-reaction library preparation kit (part code 95216-096) for generating stranded RNA-seq libraries from human, mouse, and rat (HMR) samples for Illumina NGS platforms. The kit simultaneously performs ribosomal RNA and globin mRNA depletion with integrated RNA fragmentation in a single-step, single-tube workflow to produce sequencer-ready libraries in approximately 4.5 hours. It is designed to process input RNA ranging from 1 ng (intact) or 10 ng (degraded/FFPE) up to 1 µg and is optimized to improve yields, support low-input and degraded samples, and enable rapid, directional transcriptome profiling.

Key Features:

• Configuration: 96 reactions (95216-096)
• Target species: Human, Mouse, Rat (HMR) rRNA and globin mRNA depletion
• Workflow: Single-tube integrated RNA fragmentation and depletion; ~4.5 hour protocol
• Input range: 1 ng – 1 µg total RNA (10 ng for degraded/FFPE indicated for performance)
• Output: Stranded/directional RNA-seq libraries compatible with Illumina NGS platforms

Advantages:

• Integrated rRNA and globin depletion plus fragmentation reduces hands-on steps and simplifies library prep
• Ultra-fast single-day protocol (approximately 4.5 hours) accelerates sample-to-library turnaround
• Optimized for low-input and degraded (FFPE) samples to improve library yield and transcript detection
• Proprietary enzyme chemistry and optional Bead Booster enhancements increase library yield and consistency