Quantabio sparQ HiFi PCR Master Mix 50R 95192-050

sparQ HiFi PCR Master Mix is a ready-to-use, high-fidelity, high-efficiency PCR master mix designed for amplification of DNA libraries prior to next-generation sequencing (NGS). The formulation enables library amplification while maintaining even coverage across targets, and the included primer mix permits amplification of libraries flanked by adapters containing P5 and P7 sequences required for Illumina sequencing platforms. The product is supplied as a 50-reaction kit for laboratory NGS workflows.

Key Features:

• Kit size: 50 reactions
• Manufacturer part number: 95192-050
• High-fidelity HiFi DNA polymerase engineered to minimize amplification bias
• Formulated for high-efficiency library amplification with uniform coverage across AT- and GC-rich regions
• Robust amplification from input DNA as low as 250 pg
• Includes 2X master mix and primer mix for amplification of P5/P7-adapter flanked libraries
• Recommended storage: -20°C

Advantages:

• Minimizes amplification bias through engineered HiFi DNA polymerase, supporting more uniform coverage across targets
• Increased amplification efficiency yields higher library product for downstream NGS
• Performs well on challenging AT- and GC-rich regions, improving representation of diverse sequences
• Enables robust amplification from low-input DNA (down to 250 pg), suitable for limited sample amounts