Quantabio SPARQ HIFI PCR MASTER MIX 250R 95192-250

sparQ HiFi PCR Master Mix is a high-fidelity, high-efficiency PCR master mix for NGS workflows requiring DNA library amplification prior to sequencing. The formulation uses a hot-start, proofreading HiFi DNA polymerase engineered to minimize amplification bias and reduce PCR-derived artifacts, improving library amplification efficiency and yields. The kit includes a primer mix to amplify DNA libraries flanked by adapters containing the P5 and P7 sequence elements required for Illumina sequencing platforms and supports robust amplification from input DNA as low as 250 pg.

Key Features:

• Format: 2X HiFi PCR Master Mix (kit configuration for 250 reactions)
• Engine: Hot-start, proofreading HiFi DNA polymerase engineered to minimize amplification bias
• Performance: Increased amplification efficiency resulting in higher library yields
• Coverage: Uniform coverage across AT- and GC-rich regions
• Input requirement: Robust amplification from input DNA as low as 250 pg
• Includes: Primer mix for amplification of libraries with P5 and P7 Illumina adapter sequences

Advantages:

• Higher library yields through optimized enzyme and buffer formulation
• Improved coverage uniformity across challenging AT- and GC-rich regions
• Reliable amplification from low DNA inputs (down to 250 pg) for limited samples
• Reduced PCR-derived artifacts due to hot-start, proofreading polymerase