Quantabio sparQ DNA Library Prep Kits 96R 95191-096

The sparQ DNA Library Prep Kit is a manufacturer-designed solution for rapid construction of DNA sequencing libraries from fragmented double-stranded DNA for Illumina NGS platforms. Supplied as a 96-reaction kit (96R, part code 95191-096), the kit uses a simplified two-step, single-tube workflow that converts fragmented DNA into 5'-phosphorylated, 3'-dA-tailed fragments ready for adapter ligation. Typical use cases include preparation of low-input and standard DNA samples for next-generation sequencing where reduced hands-on time and high library quality are required.

Key Features:

• Format: 96 reactions (96R).
• Part code: 95191-096.
• Workflow: simplified 2-step, single-tube protocol (under ~2.5–3 hours total prep time).
• Input DNA range: from 250 pg up to 1 µg.
• Supports PCR-free workflows from 100 ng input DNA.
• Produces 5'-phosphorylated, 3'-dA-tailed fragments ready for adapter ligation.

Advantages:

• Fast, streamlined protocol reduces total library preparation time to ~2.5–3 hours and minimizes hands-on steps.
• Increased library yields enable reliable processing of low-input samples down to 250 pg.
• High efficiency chemistry permits PCR-free workflows from 100 ng, reducing amplification-related bias.
• Single-tube reactions and combined polishing steps minimize sample transfers and potential sample loss.