Quantabio sparQ DNA Library Prep Kits 24R 95191-024

The sparQ DNA Library Prep Kit is a streamlined single-tube solution for rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina NGS platforms. The kit performs DNA end-repair and A-tailing in a combined step to produce 5'-phosphorylated, 3'-dA-tailed fragments suitable for direct adapter ligation, enabling preparation of high-quality libraries in approximately 2.5 hours. The product is supplied as a 24-reaction kit (95191-024) intended for preparing multiplexed NGS libraries from low to moderate DNA input amounts.

Key Features:

• Format: 24 reactions (95191-024)
• Total library preparation time: ~2.5 hours using a simplified 2-step single-tube protocol
• Input DNA range: 250 pg to 1 µg of fragmented double-stranded DNA
• PCR-free workflows enabled from 100 ng input DNA
• Reagents perform end-repair and 3'-dA tailing to produce 5'-phosphorylated, 3'-dA-tailed fragments for adapter ligation
• Optimized for sequencing on Illumina NGS platforms

Advantages:

• Rapid workflow with minimal hands-on time—complete libraries in ~2.5 hours
• High sensitivity for low-input samples (as low as 250 pg) and support for PCR-free workflows starting at 100 ng
• Single-tube, combined reactions reduce sample transfers and loss, simplifying laboratory workflow
• Optimized chemistry improves library yields and reduces bias for improved coverage uniformity