Quantabio sparQ DNA Frag & Library Prep Kit 24R 95194-024

The sparQ DNA Frag & Library Prep Kit is a manufacturer-specified enzymatic fragmentation and library construction kit for preparing high-quality DNA libraries for Illumina NGS platforms. The kit performs combined DNA fragmentation and end-polishing followed by adapter ligation in a simple 2-step workflow that can be completed in approximately 2.5 hours and is supplied as a 24-reaction kit (24R, part code 95194-024). It accommodates DNA input from 1 ng to 1,000 ng and enables PCR-free library workflows from 100 ng when amplification is not required.

Key Features:

• Format: 24 reactions (24R).
• Part code: 95194-024.
• Workflow: 2-step enzymatic fragmentation + adapter ligation, ~2.5 hours total.
• Input DNA range: 1 ng to 1,000 ng (1 ng–1 µg).
• Enables PCR-free workflows from 100 ng input; HiFi PCR Master Mix and Primer Mix available for amplification.
• Optimized for Illumina NGS platforms with tunable, reproducible fragmentation size range.

Advantages:

• Fast, streamlined 2-step protocol reduces hands-on time and completes library prep in ~2.5 hours.
• Wide input range (1 ng–1 µg) and PCR-free option from 100 ng support low-input and amplification-free workflows.
• Tunable fragmentation and high-fidelity amplification deliver uniform coverage and low duplication rates.
• Compatible with automation platforms to scale throughput and improve workflow reproducibility.