Quantabio repliQa HiFi ToughMix, 500R 95200-500

repliQa HiFi ToughMix is a 2× ready-to-use PCR master mix formulated for high-fidelity DNA amplification in molecular biology applications. Supplied as a 500-reaction kit, part code 95200-500, the mix combines a genetically modified high-fidelity DNA polymerase with hot-start antibodies and proprietary ToughMix chemistry to enable faster extension rates, improved sensitivity, and tolerance to common PCR inhibitors. Typical uses include routine PCR, long-range amplification, amplicon sequencing workflows, cloning, and site-directed mutagenesis.

Key Features:

• Format: 2× ready-to-use master mix
• Kit size: 500 reactions (500R)
• Part code: 95200-500
• Fidelity: >90× wild-type Taq
• Extension speed: up to 1 kb/sec (enabling 2–3× faster PCR)
• Sensitivity: effective from inputs as low as 2 pg
• Long-range capability: amplifies >24 kb genomic DNA and >40 kb λDNA

Advantages:

• High-fidelity enzyme delivers substantially reduced error rates for accurate amplicon generation.
• Accelerated extension rates reduce overall PCR run times and improve throughput.
• ToughMix chemistry provides tolerance to a wide range of PCR inhibitors, increasing success with crude or challenging templates.
• High sensitivity permits reliable amplification from low-input DNA samples.
• Supports long-range PCR targets, enabling amplification of large genomic or λDNA fragments.