Quantabio repliQa HiFi ToughMix, 25R 95200-025

repliQa HiFi ToughMix is a 2× ready-to-use PCR master mix designed for high-fidelity DNA amplification in molecular biology applications. Supplied as a 25-reaction kit, it contains a genetically modified high-fidelity DNA polymerase coupled with hot-start antibodies and proprietary ToughMix chemistry to enable faster extension rates, tolerance to common PCR inhibitors, and long-range amplification for routine PCR, cloning, amplicon sequencing, and directed mutagenesis.

Key Features:

• Format: 2× ready-to-use master mix
• Kit size: 25 reactions
• Polymerase: genetically modified high-fidelity DNA polymerase with hot-start antibodies
• Fidelity: >90× wild type Taq
• Extension rate: up to 1 kb/sec (for fragments <1 kb)
• Sensitivity: yields from inputs as low as 2 pg
• Long-range capability: amplifies >24 kb genomic DNA and >40 kb lambda DNA

Advantages:

• High fidelity enabling accurate amplification for downstream cloning and sequencing workflows
• Extreme speed that reduces PCR run times through fast extension rates
• ToughMix inhibitor tolerance suitable for challenging or crude samples
• Superior sensitivity for higher yields from low-input templates
• Capability for long-range amplification to support large-fragment targets