Quantabio qScript XLT One-Step RT-qPCR ToughMix ROX, 2000R 95133-02K

qScript XLT One-Step RT-qPCR ToughMix ROX is a ready-to-use, 2X master mix for one‑tube reverse transcription quantitative PCR (RT‑qPCR) using hydrolysis probe detection. The formulation performs first‑strand cDNA synthesis and PCR amplification in the same tube and is intended for sensitive quantification of RNA templates, including RNA viruses and low‑abundance targets, in high‑throughput gene expression and diagnostic workflows. The supplied configuration provides 2000 reactions (1 x 20 mL) and includes ROX passive reference dye for instruments requiring a reference.

Key Features:

• Format: 2X ready-to-use One-Step RT-qPCR master mix
• Pack size: 2000 x 20 µL reactions (1 x 20 mL)
• Passive reference: ROX included (optimized titration)
• Enzymes included: qScript XLT reverse transcriptase, RNase inhibitor and hot-start Taq DNA polymerase
• Contains stabilizers, optimized buffer and inert plate-loading dye

Advantages:

• High sensitivity suitable for quantifying RNA viruses and low‑abundance RNA targets
• Tough-tested formulation tolerant to a wide range of PCR inhibitors
• Integrated ROX reference dye normalizes signal variation on ROX‑requiring real-time cyclers
• Single‑tube one‑step workflow reduces handling and supports high‑throughput experiments