Quantabio qScript XLT cDNA SuperMix,100R 95161-100

qScript XLT cDNA SuperMix is a stabilized, single-tube 5X concentrated master mix for first-strand cDNA synthesis, formulated for two-step RT-PCR and RT-qPCR applications. It contains an engineered qScript XLT M-MLV reverse transcriptase with reduced RNase H activity and a pre-mixed combination of random hexamer and oligo(dT) primers to produce unbiased representation of 5′ and 3′ RNA sequences ≤1 kb. The product is provided as a 100-reaction pack (100R) with catalog code 95161-100 and is intended for sensitive cDNA synthesis from limiting or complex RNA templates prior to downstream PCR or quantitative PCR.

Key Features:

• Pack size: 100 reactions (100R); catalog number 95161-100
• Stabilized 5X concentrated single-tube master mix
• Contains engineered qScript XLT M-MLV reverse transcriptase with reduced RNase H activity
• Includes optimized mix of random hexamer and oligo(dT) primers for equal 5′/3′ representation ≤1 kb
• Broad linear dynamic detection range and improved cDNA yields (manufacturer reports 2–3× yield improvement over older enzyme technologies)

Advantages:

• Simplifies reaction setup by providing a single, 5X concentrated master mix that reduces pipetting steps
• Improves sensitivity and yield for low-abundance or difficult RNA targets via an engineered reverse transcriptase and enhanced template binding
• Delivers balanced representation across transcript ends for more accurate two-step RT-PCR and RT-qPCR results