Quantabio qScript UltraFlex 100R 95215-100

qScript UltraFlex 100R is a first-strand cDNA synthesis kit designed for reverse transcription of RNA into single-stranded cDNA for downstream RT-PCR and RT-qPCR workflows. The kit uses a next-generation, RNase H–deficient reverse transcriptase engineered for improved thermostability and processivity, supports flexible priming methods (oligo-dT, random primers, and gene-specific primers), and is intended for molecular biology applications requiring rapid, high-yield cDNA synthesis.

Key Features:

• Format: Kit for 100 reactions
• Engine: RNase H–deficient reverse transcriptase with enhanced thermostability (activity up to 65°C)
• Flexible priming: Compatible with oligo-dT, random primers, and gene-specific priming
• Speed: 10-minute reaction time (rapid first-strand synthesis)
• Performance: Enables reverse transcription of long transcripts (up to ~20 kb) and improved resistance to common PCR inhibitors
• Contents: Includes required components for first-strand cDNA synthesis except RNA template and gene-specific primers

Advantages:

• Improved thermostability enables efficient reverse transcription through strong RNA secondary structures
• Fast protocol reduces hands-on and total processing time for cDNA preparation
• Flexible priming supports diverse experimental designs (oligo-dT, random, or gene-specific)
• Enhanced inhibitor resistance and high sensitivity improve yield from challenging or limited samples
• Capability to synthesize long cDNA products supports assays targeting large transcripts