Quantabio qScript Ultra SuperMix, 100R 95217-100

qScript Ultra SuperMix is a single-tube cDNA synthesis master mix for first-strand reverse transcription, supplied as a 100-reaction kit (100R, catalog 95217-100). The mix contains all required components for first-strand cDNA synthesis except the RNA template and is directly compatible with downstream 2-step RT-qPCR or RT-PCR workflows. It is intended for gene expression analysis and RNA pathogen quantification studies where rapid, reproducible first-strand synthesis and unbiased representation of RNA sequences are required.

Key Features:

• Format: single-tube SuperMix containing all components for first-strand cDNA synthesis except RNA template
• Pack size: 100 reactions (100R).
• Manufacturer part code: 95217-100
• Thermostability: engineered RT activity up to 65 °C
• Speed: 10 minute reaction time with a total protocol time of ~13 minutes
• Performance: wide dynamic range maintaining linearity from 2.5 µg to 1 pg total RNA
• Compatibility: directly compatible with downstream 2-step RT-qPCR or RT-PCR

Advantages:

• Enhanced thermostability enables efficient reverse transcription through challenging RNA secondary structures.
• Fast protocol reduces hands-on and run time with first-strand synthesis completed in ~10 minutes.
• High sensitivity and broad dynamic range preserve cDNA linearity across a wide span of RNA inputs.
• Resistance to common PCR inhibitors improves performance with difficult sample types.
• Single-tube format simplifies setup and reduces pipetting variability.