Quantabio Qscript lyo 1-Step, 24R 95198-024

Qscript lyo 1-Step is a lyophilized, single-reaction master mix designed for one-step RT-qPCR of RNA targets. Supplied in an eight-strip PCR tube format with 24 reactions per pack, the reagent contains a hot-start thermostable DNA polymerase and a genetically engineered reverse transcriptase to enable sensitive, reproducible RT-qPCR with hydrolysis probes. Typical use cases include sensitive detection and quantification of RNA targets and multiplex assays of up to five targets where ambient-temperature shipping and simplified setup are required.

Key Features:

• Format: Lyophilized single-reaction master mix in eight-strip PCR tubes (24 reactions)
• Enzymes: Hot-start thermostable polymerase and genetically engineered reverse transcriptase included
• Sensitivity: Detects down to 0.5 pg RNA; dynamic range 0.5–500 pg RNA
• Multiplexing: Supports up to 5 targets per reaction
• Stability: Ambient storage up to 9 months; refrigerated (4°C) storage up to 2 years
• Compatibility: Designed for RT-qPCR with hydrolysis probes and compatible with compact rapid cyclers

Advantages:

• Eliminates the need for a cold chain for shipping and short-term storage, simplifying logistics
• Pre-dispensed single-tube format reduces hands-on setup time and lowers cross-contamination risk
• High sensitivity and broad dynamic range enable detection of low-abundance RNA targets
• Multiplex capability (up to five targets) supports efficient multi-target assays in a single reaction