Quantabio qScript Flex cDNA Synthesis Kit, 25R 95049-025

The qScript Flex cDNA Synthesis Kit is a flexible first‑strand cDNA synthesis system designed for two‑step RT‑PCR workflows. It provides separate, optimized components to support multiple priming strategies (oligo‑dT, random hexamers, or gene‑specific primers) and generates high‑quality first‑strand cDNA products up to 12 kb in length for quantitative real‑time or end‑point RT‑PCR applications. The kit is supplied for 25 reactions and is intended for molecular biology research use.

Key Features:

• Pack size: 25 reactions
• Supports priming with oligo‑dT, random hexamers, or gene‑specific primers (GSP)
• First‑strand cDNA product lengths up to 12 kb
• Dynamic input range: from 10 pg to 4 µg total RNA
• 5X master mix formulation containing buffer, dNTPs and MgCl2 plus separate reverse transcriptase/RNase inhibitor mix

Advantages:

• Flexible priming options enable tailored cDNA synthesis strategies for diverse targets and workflows
• Wide dynamic range and high sensitivity allow reliable cDNA generation from limiting to abundant RNA inputs
• Optimized separate components and 5X master mix simplify reaction assembly and promote robust, reproducible first‑strand synthesis compatible with downstream RT‑PCR