Quantabio qScript Flex cDNA Synthesis Kit, 100R 95049-100

qScript Flex cDNA Synthesis Kit is a first-strand cDNA synthesis system for converting RNA to cDNA for downstream 2-step RT-qPCR or RT-PCR applications. The kit provides separated components to support flexible priming strategies (oligo(dT), random hexamer or gene-specific primers) and includes a 5X reaction mix plus reverse transcriptase and a priming enhancer to maximize cDNA yield. The 100-reaction format is configured to produce robust first-strand cDNA products up to 12 kb depending on priming method and RNA quality.

Key Features:

• Format: 100 reactions
• Supports priming: oligo(dT), random hexamers, and gene-specific primers (user-defined)
• 5X qScript Flex Reaction Mix with Mg2+, dNTPs and stabilizers
• Reverse transcriptase supplied as separate component
• cDNA product length: up to 12 kb (dependent on priming and RNA quality)
• Input RNA dynamic range: from 10 pg to 4 µg total RNA
• Output compatible with downstream 2-step RT-qPCR and conventional RT-PCR

Advantages:

• Flexible priming options enable tailored cDNA synthesis for diverse targets and applications
• Priming enhancer and optimized mix maximize cDNA yield from limiting or compromised samples
• Wide input RNA dynamic range supports both low-abundance and abundant templates
• Produces long cDNA products (up to 12 kb) when using oligo(dT) or gene-specific priming, enabling long-target analysis
• Prepared components simplify reaction setup and provide reproducible first-strand synthesis compatible with common downstream assays