Quantabio qScript cDNA Synthesis Kit, 100R 95047-100

The qScript cDNA Synthesis Kit is a two-component reverse transcription system for synthesis of first-strand cDNA for two-step RT-qPCR and conventional RT-PCR applications. The kit provides a pre‑prepared qScript Reaction Mix and qScript reverse transcriptase to generate cDNA directly compatible with downstream qPCR chemistries for amplified fragments ≤1 kb. Supplied for 100 reactions (95047-100), it is intended for sensitive quantification of RNA across a wide input range in molecular biology research.

Key Features:

• Pack size: 100 reactions (95047-100)
• Two-component system: qScript Reaction Mix + qScript reverse transcriptase
• Pre-mixed oligo(dT) and random primers for balanced 5′/3′ representation
• Performance linear across RNA inputs from 10 pg to 1 µg total RNA
• Resulting cDNA compatible with qPCR or conventional RT-PCR for fragments ≤1 kb
• Reagent stability: performance unaffected by >20 freeze–thaw cycles

Advantages:

• Sensitive and linear detection across a broad RNA input range, enabling work with both low and abundant samples
• Pre‑optimized master mix and primer combination give even 5′/3′ representation for accurate gene expression quantification
• Two-component format simplifies setup while maintaining reagent stability through repeated freeze–thaw handling
• Resulting cDNA is directly compatible with standard qPCR chemistries, streamlining two-step RT-qPCR workflows