Quantabio qScript cDNA SuperMix, 25R 95048-025

qScript cDNA SuperMix is a stabilized, 5X concentrated master mix for first‑strand cDNA synthesis designed for two‑step RT‑PCR and RT‑qPCR applications. The SuperMix contains pre‑blended random and oligo(dT) primers and a ribonuclease inhibitor, and is formulated to deliver sensitive first‑strand cDNA synthesis of RNA sequences ≤1 kb across a broad linear dynamic range from limiting (10 pg) to plentiful (1 µg) total RNA inputs. The product provides a single‑tube, ready‑to‑use solution for producing cDNA for real‑time and conventional RT‑PCR workflows.

Key Features:

• Pack size: 25 reactions
• Manufacturer part number: 95048-025
• Formulation: 5X concentrated master mix
• Target cDNA length: optimized for RNA sequences ≤1 kb
• Input RNA dynamic range: 10 pg to 1 µg total RNA
• Includes: buffer, dNTPs, MgCl2, proprietary mix of random + oligo(dT) primers, RNase inhibitor, qScript reverse transcriptase, stabilizers

Advantages:

• Sensitive first‑strand cDNA synthesis suitable for low‑input and high‑input RNA samples (10 pg–1 µg).
• Single‑tube, 5X concentrated SuperMix minimizes pipetting steps and improves assay accuracy.
• Pre‑blended primer mix delivers unbiased representation of 5′ and 3′ sequences for targets ≤1 kb.
• Contains RNase inhibitor and stabilized components to protect RNA template and support reliable cDNA synthesis for downstream RT‑PCR or RT‑qPCR.