Quantabio qScript 1-Step Virus ToughMix low ROX, 500R 95212-500

qScript 1-Step Virus ToughMix low ROX is a 2X ready-to-use master mix for rapid one-step reverse transcription quantitative PCR (RT-qPCR) detection of RNA viruses and other RNA targets. The formulation performs first-strand cDNA synthesis and PCR amplification in a single closed tube and is engineered for high sensitivity with tolerance to common PCR inhibitors, enabling reliable detection from challenging sample types. Supplied in a 500R format (500 reactions) as catalog number 95212-500 with Low-ROX passive reference dye, it is intended for probe-based (e.g., TaqMan) RT-qPCR assays on instruments requiring low ROX normalization.

Key Features:

• Format: 2X ready-to-use master mix
• Kit size: 500 reactions (500 x 20 µL) — 95212-500
• Low ROX passive reference dye included
• Contains qScript XLT reverse transcriptase, RNase inhibitor, hot-start DNA polymerase, nucleotides, MgCl2 and stabilizers
• Compatible with probe-based one-step RT-qPCR for viral RNA detection
• Tolerant to common PCR inhibitors; supports multiplexing up to four targets
• Storage: stable when stored at -20°C (store protected from light)

Advantages:

• High sensitivity optimized for low viral RNA inputs enabling reliable detection of RNA viruses
• Tough-tested formulation tolerates sample-derived inhibitors for robust performance on challenging specimens
• Single-tube one-step workflow reduces handling and contamination risk while streamlining assay setup
• Low-ROX formulation matches instruments that require reduced passive reference dye for accurate normalization