Quantabio qScript 1-Step Virus TM Kit, 500R 95131-500

qScript 1-Step Virus ToughMix is a 2X, ready-to-use master mix for one-step, single-tube reverse transcription quantitative PCR (RT-qPCR) for rapid detection of RNA viruses including Influenza A, Influenza B and SARS-CoV-2. The formulation is optimized for high sensitivity with improved performance on low viral RNA inputs and is compatible with hydrolysis probe (TaqMan®) assays in singleplex or multiplex formats. The mix incorporates a thermostable reverse transcriptase with reduced RNase H activity and RT Hot-Start functionality to enable higher-temperature reverse transcription (50–55°C) for improved primer specificity and disruption of RNA secondary structure.

Key Features:

• Format: 2X ready-to-use master mix for one-step RT-qPCR
• Intended targets: RNA viruses such as Flu-A, Flu-B and SARS-CoV-2
• Kit size/packaging: 500 reactions (500R); part code 95131-500
• Enzyme components: qScript XLT reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase
• Detection chemistry: compatible with dual-labeled hydrolysis probes (TaqMan®); supports multiplexing

Advantages:

• Optimized for superior sensitivity enabling reliable detection from low viral RNA inputs
• RT Hot-Start and elevated RT temperatures (50–55°C) improve primer specificity and resolve RNA secondary structure
• Tolerant to a range of PCR inhibitors, supporting robust performance on challenging samples
• Ready-to-use 2X formulation simplifies setup for single-tube one-step RT-qPCR and supports multiplex assays