Quantabio qScript 1-Step SYBR Green qRT-PCR Kit L-ROX, 200R 95089-200

qScript 1-Step SYBR Green qRT-PCR Kit L-ROX is a one-tube, one-step real-time RT-qPCR master mix for quantitative detection of RNA templates using SYBR Green I dye. The kit performs reverse transcription and PCR in the same reaction vessel and includes L-ROX reference dye for normalization. Supplied as a 200 reaction kit, it is formulated for sensitive RNA detection and supports both conventional and fast thermal cycling for gene expression and diagnostic research applications.

Key Features:

• Format: 200 reactions
• Chemistry: SYBR Green I dye detection
• Includes L-ROX passive reference dye
• Reverse transcriptase: RNase H(+) M-MLV reverse transcriptase
• Polymerase: ultra-pure antibody hot-start Taq DNA polymerase
• Compatible with conventional and fast cycling protocols

Advantages:

• High sensitivity RNA detection enabled by engineered RNase H(+) M-MLV reverse transcriptase.
• Improved specificity and reduced non-specific amplification due to ultra-pure antibody hot-start polymerase.
• Normalized fluorescence signals across wells and instruments provided by included L-ROX reference dye.
• Flexible workflow compatibility with both standard and fast qPCR thermal cycling protocols.