Quantabio QSCRIPT 1-STEP RT-QPCR 95057-200

qScript One-Step RT-qPCR Kit is a 2X master mix system for performing reverse transcription and quantitative PCR in a single tube using probe-based detection chemistries (for example, TaqMan 5'-hydrolysis probes). cDNA synthesis and PCR amplification are carried out without opening the reaction tube, enabling sensitive RT-qPCR of RNA templates for gene expression analysis, pathogen detection, and other molecular biology applications. The product is supplied in a 200 x 50 µL reaction format.

Key Features:

• Format: 2X RT-qPCR master mix (one-step, single-tube)
• Pack size: 200 x 50 µL reactions (95057-200)
• Detection chemistry: designed for probe-based assays (e.g., TaqMan 5'-hydrolysis probes)
• Enzyme composition: proprietary reverse transcriptase plus hot-start DNA polymerase
• Product variants: available with ROX or Low-ROX reference dye options

Advantages:

• One-step workflow combines reverse transcription and qPCR in the same tube, reducing hands-on time and contamination risk
• Optimized buffer and enzyme formulation deliver high sensitivity and specificity for low-copy RNA targets
• Compatible with standard and fast thermal cycling protocols, offering workflow flexibility
• ROX-containing and Low-ROX variants enable use across instruments that require an internal reference dye