Quantabio PerfeCTa SYBR Green SuperMix for IQ, 500R 95053-500

PerfeCTa SYBR Green SuperMix for iQ is a 2X concentrated, ready-to-use qPCR reaction mix formulated for SYBR Green I detection on real-time instruments including Bio-Rad iQ platforms. The mix is pre-blended with reference dye and an antibody-mediated hot-start AccuStart Taq DNA polymerase to enable room-temperature reaction setup, efficient target amplification, and strong fluorescent signal for detection of low-abundance or small amplicons. Primary use cases are quantitative PCR assays that employ SYBR Green I chemistry for gene expression analysis and other dsDNA detection applications.

Key Features:

• Format: 2X concentrated reaction mix, pre-blended with reference dye
• Enzyme: AccuStart antibody hot-start Taq DNA polymerase
• Dye: High-concentration SYBR Green I stabilization for robust optical signal
• Pack size / volume: 500 reactions (500 x 50 µL; supplied as 10 x 1.25 mL)
• Part number: 95053-500 (for iQ)

Advantages:

• Simplifies setup and reduces pipetting steps through a 2X, pre-mixed formulation
• Enhanced specificity and room-temperature assembly enabled by antibody hot-start AccuStart enzyme
• Optimized buffer and stabilizers for maximum PCR efficiency, sensitivity, and a robust fluorescent signal with small or low-abundance targets
• Pre-blended reference dye ensures compatibility with instruments requiring passive reference normalization