Quantabio PerfeCTa SYBR Green FastMix, ROX 5000 95073-05K

PerfeCTa SYBR Green FastMix, ROX 5000 is a ready-to-use SYBR Green‑based real-time PCR master mix formulated for fast cycling qPCR applications. The kit contains an optimized reaction buffer with MgCl2, dNTPs, AccuFast hot‑start Taq DNA polymerase, SYBR Green I dye and ROX passive reference dye, and is supplied in a format sufficient for 5000 × 20 µL reactions. It is intended for sensitive, specific detection and quantitation of double‑stranded DNA targets using instruments that require ROX normalization.

Key Features:

• Format: Master mix containing AccuFast hot‑start Taq DNA polymerase
• Detection chemistry: SYBR Green I dye with ROX passive reference dye
• Reaction capacity: 5000 reactions (20 µL each)
• Composition includes optimized reaction buffer and dNTPs with MgCl2
• Intended for fast cycling qPCR and instruments requiring ROX
• Storage: −20°C

Advantages:

• Optimized formulation for high sensitivity and specificity in SYBR Green assays
• AccuFast hot‑start enzyme minimizes room‑temperature activity and enables rapid activation at 95°C for efficient extension
• Includes ROX passive reference dye for signal normalization on ROX‑requiring instruments
• Ready‑to‑use mix reduces hands‑on preparation and supports fast cycling workflows