Quantabio PerfeCTa SYBR Green FastMix, L-ROX 1250 95074-012

PerfeCTa SYBR Green FastMix, L-ROX is a ready-to-use 2X master mix for real-time PCR (qPCR) that provides SYBR Green–based detection with an included L-ROX passive reference dye. The formulation contains proprietary buffer components, stabilizers and AccuFast hot-start Taq DNA polymerase to deliver high sensitivity, specificity and efficient amplification under either accelerated (fast) or conventional cycling conditions. Primary use cases are quantitative PCR assays using intercalating-dye detection for DNA targets in molecular biology research applications.

Key Features:

• 2X concentrated, ready-to-use master mix
• Includes L-ROX passive reference dye
• Formulation supports 1250 x 20 µL reactions (10 × 1.25 mL)
• Contains AccuFast hot-start Taq DNA polymerase
• Optimized proprietary buffer and stabilizers for fast and standard cycling

Advantages:

• High assay sensitivity and specificity through AccuFast hot-start enzyme and optimized buffer system
• Compatible with fast or standard thermal cycling, enabling accelerated qPCR workflows
• Ready-to-use 2X format reduces pipetting steps and simplifies reaction setup
• Included L-ROX reference dye provides internal normalization for instruments requiring ROX