Quantabio PerfeCTa SYBR Green FastMix, 5000R 95072-05K

PerfeCTa SYBR Green FastMix is a ready-to-use, 2X concentrated SYBR Green master mix formulated for fast or standard real-time PCR amplification and fluorescence-based detection of double-stranded DNA. The formulation contains AccuStart II hot-start Taq DNA polymerase, SYBR Green I dye, reaction buffer with optimized dNTPs and MgCl2, stabilizers, and a titrated reference dye where applicable. The product is supplied as a 5000 reaction kit (50 mL total at 20 µL reactions) for high-throughput qPCR workflows and is intended for molecular biology research applications using SYBR Green–based detection.

Key Features:

• Format: 2X concentrated master mix, single-tube ready-to-use formulation
• Enzyme: AccuStart II hot-start Taq DNA polymerase
• Dye: SYBR Green I included for dsDNA detection
• Kit size: 5000 reactions (1 x 50 mL for 20 µL reactions)
• Compatibility: Supports fast and standard thermal cycling protocols
• Storage: Store at -25°C to -15°C protected from light

Advantages:

• Minimizes pipetting and assembly steps through 2X concentrated single-tube formulation, improving workflow accuracy
• Ultrapure AccuStart hot-start enzyme enhances specificity and sensitivity by reducing non-specific amplification
• FastMix chemistry enables accelerated thermal cycling to reduce time-to-result while retaining amplification performance
• Proprietary anti-foaming and stabilizing additives support efficient mixing and reagent stability during handling and storage