Quantabio PerfeCTa SYBR Green FastMix, 1250R 95072-012

PerfeCTa SYBR Green FastMix is a ready-to-use, 2X concentrated master mix for real-time PCR (qPCR) applications using SYBR Green detection. The formulation contains AccuStart/AccuFast hot-start Taq DNA polymerase, SYBR Green I dye, proprietary buffer and stabilizers, and is supplied as 10 x 1.25 mL (1250 x 20 µL reactions). It is designed to deliver high sensitivity and specificity for quantitative amplification under both fast and standard thermal cycling conditions.

Key Features:

• Format: 2X concentrated ready-to-use master mix
• Pack size: 10 x 1.25 mL (total 12.5 mL) — 1250 x 20 µL reactions
• Detection chemistry: SYBR Green I dye included
• Enzyme: AccuStart/AccuFast hot-start Taq DNA polymerase
• Performance: supports fast and standard thermal cycling; contains proprietary buffer and stabilizers
• Formulation features: anti-foaming additive and titrated reference dye (where applicable)

Advantages:

• Minimizes pipetting steps and simplifies reaction setup through a 2X concentrated, ready-to-use format
• Provides superior assay sensitivity and specificity via ultrapure hot-start Taq polymerase and optimized buffer chemistry
• Compatible with accelerated (fast) and conventional cycling protocols for flexible throughput
• Supports efficient vortex mixing and reduced foaming due to proprietary anti-foaming formulation