Quantabio Perfecta qPCR ToughMix UNG L-ROX, 5000R 95140-05K

PerfeCTa qPCR ToughMix UNG L-ROX is a 2X concentrated, ready-to-use real-time PCR master mix formulated for probe-based qPCR assays. It contains uracil-N-glycosylase (UNG) to prevent carry-over contamination and a low-ROX passive reference dye for instruments requiring L-ROX normalization. The mix is intended for high-sensitivity quantitation with TaqMan-style hydrolysis probes across a wide range of sample types and challenging matrices.

Key Features:

• Format: 2X concentrated, ready-to-use master mix for qPCR
• Carry-over prevention: contains uracil-N-glycosylase (UNG)
• Passive reference dye: L-ROX included
• Enzyme: hot-start engineered Taq DNA polymerase (AccuStart II technology)
• Pack size: 5000 x 20 µL reactions (1 x 50 mL)
• Compatibility: optimized for probe-based (TaqMan) qPCR assays
• Inhibitor tolerance: formulation includes ToughMix additives for challenging samples

Advantages:

• Reduces risk of amplicon carry-over contamination through inclusion of UNG
• Provides consistent signal normalization on instruments requiring L-ROX, improving inter-run comparability
• Delivers high sensitivity and efficiency with an engineered hot-start polymerase and inhibitor-tolerant formulation
• Supplies a large-volume kit suitable for high-throughput workflows (5,000 reactions)