Quantabio Perfecta qPCR ToughMix UNG, 5000R 95138-05K

PerfeCTa qPCR ToughMix UNG is a 2X concentrated, ready-to-use real-time PCR master mix formulated for probe-based qPCR assays. It contains uracil-N-glycosylase (UNG) and all reaction components except primers, probe(s), and template, and is designed to enable robust amplification of DNA from challenging sample matrices and probe chemistries (including TaqMan® hydrolysis probes). The product is supplied as a bulk 5000-reaction format for high-throughput applications.

Key Features:

• Format: 2X concentrated ready-to-use qPCR master mix
• Kit size: 5000 x 20 µL reactions (1 x 50 mL)
• Contains: uracil-N-glycosylase (UNG) to prevent carry-over contamination
• Includes AccuVue blue tracer dye to simplify reaction assembly
• Stability: stable for 2 years at −20°C; may be stored 2–8°C up to 6 months
• Compatible with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes

Advantages:

• Neutralizes common PCR inhibitors for reliable performance with environmental, plant, animal, and complex sample types
• UNG inclusion reduces risk of amplification from carry-over dU-containing PCR products
• AccuVue tracer dye and anti-foam formulation simplify reaction setup and improve pipetting accuracy
• Exceptional reagent stability that tolerates multiple freeze–thaw cycles and extended storage at ambient/refrigerated conditions