Quantabio Perfecta qPCR ToughMix UNG, 1250R 95138-012

PerfeCTa qPCR ToughMix UNG is a 2X concentrated, ready-to-use reaction cocktail formulated for probe-based real-time quantitative PCR. It contains uracil-N-glycosylase (UNG) to prevent carry-over contamination, a hot-start DNA polymerase, optimized buffer, dNTPs and stabilizers, and is designed for reliable amplification from challenging starting materials such as clinical, environmental, plant, or food specimens. Primary use cases include sensitive, inhibitor-tolerant qPCR assays requiring contamination control for diagnostic and research applications.

Key Features:

• Packaging for 1250 × 20 µL reactions (10 × 1.25 mL)
• 2X concentrated ready-to-use reaction mix containing UNG
• Includes hot-start DNA polymerase, optimized MgCl2 and dNTP concentrations
• Formulation engineered to neutralize common PCR inhibitors
• Demonstrated reagent stability (resistant after multiple freeze–thaw cycles and stable at ambient temperature for defined periods)

Advantages:

• Integrated UNG reduces risk of amplification from prior dU-containing PCR products, improving assay integrity
• ToughMix formulation tolerates common PCR inhibitors, enabling reliable performance with complex sample types
• Ready-to-use 2X format simplifies setup and reduces hands-on preparation
• Robust stability supports consistent performance across handling and storage conditions