Quantabio Perfecta qPCR ToughMix ROX, 5000R 95113-05K

PerfeCTa qPCR ToughMix ROX is a 2X concentrated, ready-to-use real-time PCR master mix formulated for robust amplification of DNA templates and probe-based qPCR. The formulation overcomes many PCR inhibitors commonly found in crude or complex sample types and is compatible with fluorogenic probe chemistries (including TaqMan® probes) for routine gene expression, DNA quantitation, and presence/absence assays. The product is supplied in a 50 mL format sufficient for 5000 × 20 µL reactions and includes a ROX passive reference dye for instrument normalization.

Key Features:

• 2X ready-to-use reaction buffer containing optimized MgCl2, dNTPs, and stabilizers
• Includes hot-start DNA polymerase and AccuVue blue qPCR dye
• Contains ROX passive reference dye for instrument normalization
• Packaged as 1 × 50 mL, sufficient for 5000 × 20 µL reactions
• Exceptional reagent stability: uninhibited after 20× freeze–thaw and stable for 30 days at 22°C

Advantages:

• Neutralizes common PCR inhibitors to enable reliable performance with environmental, plant, clinical, or complex food matrices
• High reagent stability supports repeated freeze–thaw and temporary storage at ambient temperature without loss of performance
• Ready-to-use 2X formulation simplifies reaction setup and reduces hands-on preparation
• Compatible with probe-based detection (including TaqMan probes) and both fast and conventional PCR cycling protocols