Quantabio Perfecta qPCR ToughMix ROX, 1250R 95113-012

PerfeCTa qPCR ToughMix ROX is a ready-to-use, 2X concentrated real-time PCR master mix formulated for hydrolysis probe (e.g., TaqMan) qPCR assays. Supplied as a bulk kit sufficient for 1250 reactions (20 µL each), the product contains an ultra-pure hot-start DNA polymerase, optimized buffer, dNTPs, and a ROX passive reference dye to enable normalized fluorescent signal detection. It is intended for sensitive, high-throughput quantitative PCR applications across research workflows requiring reliable amplification and fluorescence normalization.

Key Features:

• Format: 2X concentrated master mix for qPCR
• Pack size: sufficient for 1250 reactions (20 µL each); supplied as 10 × 1.25 mL
• Passive reference dye: ROX included
• Enzyme: AccuStart II ultra-pure hot-start Taq DNA polymerase
• Detection chemistry: compatible with hydrolysis probes (TaqMan-style)
• Includes AccuVue tracer/loading dye and optimized MgCl2 and dNTP concentrations

Advantages:

• Tolerance to common PCR inhibitors for reliable performance with challenging sample matrices
• High sensitivity and efficiency for probe-based qPCR enabled by AccuStart II hot-start enzyme
• Pre-mixed, concentrated format reduces setup time and risk of pipetting errors for high-throughput workflows
• ROX reference dye provides consistent fluorescence normalization for accurate quantification