Quantabio Perfecta qPCR ToughMix L-ROX, 5000R 95114-05K

PerfeCTa qPCR ToughMix L-ROX is a 2X concentrated, ready-to-use qPCR reaction cocktail designed for PCR amplification of DNA templates. The formulation overcomes many known inhibitors often present in crude samples from environmental, plant, or animal tissues and is compatible with fluorogenic probe chemistries, including TaqMan hydrolysis probes. The mix is pre-formulated with a low-ROX passive reference dye (L-ROX), AccuStart II hot-start DNA polymerase, optimized buffer, dNTPs, and the AccuVue tracer dye to simplify reaction assembly. Product configuration: 5000R, catalog/part code 95114-05K.

Key Features:

• Format: 2X concentrated ready-to-use qPCR reaction mix
• Includes AccuStart II hot-start DNA polymerase
• Contains AccuVue tracer dye and low-ROX (L-ROX) passive reference dye
• Compatible with TaqMan® hydrolysis probes and other fluorogenic probe chemistries
• Stability: stable for 2 years at -20°C; may be stored unfrozen at 2–8°C for up to 6 months
• Pack size/configuration: 5000R (5000 x 20 µL reactions; 1 × 50 mL)
• Includes anti-foam technology for efficient vortex mixing and resistance to freeze–thaw

Advantages:

• Neutralizes common PCR inhibitors for reliable amplification from challenging sample types
• High reagent stability (resistant to multiple freeze–thaw cycles and room-temperature exposure)
• Ready-to-use 2X format with tracer dye reduces setup errors and speeds assay preparation
• Optimized for high sensitivity and efficiency with probe-based qPCR assays