Quantabio Perfecta qPCR ToughMix, 250R 95112-250

PerfeCTa qPCR ToughMix is a 2X concentrated, ready-to-use qPCR master mix formulated for real-time PCR amplification of DNA templates. It contains optimized buffer components, dNTPs, MgCl2, a hot-start DNA polymerase (AccuStart enzyme technology), an included qPCR dye, and stabilizers, and is designed to neutralize common PCR inhibitors encountered in crude or complex sample matrices. Supplied as 250 reactions (250R) in the 95112-250 configuration (two 1.25 mL tubes), it is intended for applications requiring robust, inhibitor-resistant probe-based qPCR performance across clinical, environmental, plant, or food-tested samples.

Key Features:

• Format: 2X concentrated ready-to-use master mix
• Contents include optimized buffer, MgCl2, dNTPs, AccuStart hot-start DNA polymerase, qPCR dye, and stabilizers
• Pack size: 250 reactions supplied as 2 × 1.25 mL (95112-250)
• Tolerance to PCR inhibitors for use with crude or complex sample matrices
• Enhanced reagent stability (stable after multiple freeze–thaw cycles and at ambient conditions for extended periods) and anti-foam formulation

Advantages:

• Robust inhibitor tolerance enables reliable amplification from challenging sample types without extensive sample cleanup
• Ready-to-use 2X format reduces pipetting steps and simplifies assay setup
• AccuStart hot-start enzyme and optimized buffer deliver high sensitivity and PCR efficiency
• Anti-foam and stabilizer formulation support consistent mixing and reagent longevity