Quantabio PerfeCTa qPCR SuperMix ROX, 500R 95051-500

PerfeCTa qPCR SuperMix ROX is a 2X, ready-to-use master mix for real-time quantitative PCR that contains all reaction components except primers, probe(s), and template. It is formulated for use with probe-based assays (TaqMan®/TaqMan MGB chemistry) and includes a ROX passive reference dye for fluorescence normalization. The mix is designed to deliver high efficiency and sensitivity for qPCR workflows and employs a hot-start Taq DNA polymerase to control activation prior to cycling.

Key Features:

• Format: 2X concentrated master mix, ready-to-use
• Contains ROX passive reference dye for normalization
• Includes AccuStart Taq DNA polymerase with antibody-based hot-start
• Intended for use with TaqMan® or TaqMan MGB probe chemistry
• Pack size: 500 reactions (500 x 50 µL reactions; 10 x 1.25 mL)

Advantages:

• Optimized buffer and stabilizers provide high PCR efficiency and sensitivity for low-abundance targets
• Antibody-mediated hot-start enzyme reduces non-specific amplification and improves specificity
• ROX reference dye enables normalization of fluorescent signal across runs and instruments
• Ready-to-use 2X formulation simplifies setup and reduces pipetting steps