Quantabio PerfeCTa qPCR SuperMix, 500R 95050-500

PerfeCTa qPCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail formulated for real-time quantitative PCR (qPCR). The mix contains all components required for probe-based qPCR except primers, probe(s), and template, and is optimized to deliver high PCR efficiency, sensitivity, and robust fluorescent signal with TaqMan® or TaqMan MGB probe chemistries. The product supports reliable detection and discrimination in applications such as gene expression analysis and SNP assays.

Key Features:

• Format: 2X concentrated, ready-to-use master mix
• Reactions per kit: 500 reactions (50 µL each; supplied as 10 × 1.25 mL aliquots)
• Probe chemistry compatibility: TaqMan® and TaqMan MGB probes
• Polymerase: AccuStart hot-start Taq DNA polymerase with monoclonal antibody inhibition
• Heat activation: Antibody-mediated hot-start released by heat activation (2 minutes at 95°C)

Advantages:

• Room-temperature reaction assembly enabled by antibody-mediated hot-start polymerase for convenience and reduced setup variability
• Optimized buffer and stabilizers provide high sensitivity and strong fluorescent signal for probe-based qPCR
• Enhanced specificity reduces cross-reactivity between homologous sequences, improving SNP detection and discrimination
• Supplied at 2X concentration to simplify reaction setup and minimize pipetting steps