Quantabio PerfeCTa qPCR FastMix, UNG ROX 5000R 95077-05K

PerfeCTa qPCR FastMix, UNG ROX is a 2X concentrated, ready-to-use qPCR reaction cocktail formulated for rapid and conventional real-time PCR using TaqMan probe chemistries. The mix contains AccuFast Taq DNA polymerase, uracil-N-glycosylase (UNG) to prevent carry-over contamination, and ROX passive reference dye for instruments that require normalization. It is optimized with proprietary buffers and stabilizers to deliver high PCR efficiency, sensitivity, and robust fluorescent signal during fast cycling and reduced reaction volumes for quantitative PCR workflows.

Key Features:

• 2X concentrated, ready-to-use master mix
• Includes uracil-N-glycosylase (UNG) to prevent carry-over contamination
• Formulated with ROX passive reference dye for instruments requiring normalization
• Contains AccuFast Taq DNA polymerase with heat activation (20 seconds at 95°C)
• Designed for 5000 reactions (5000R) — catalog identifier 95077-05K

Advantages:

• Enables fast cycling and reduced reaction volumes while maintaining high efficiency and sensitivity
• UNG inclusion eliminates amplification from dU-containing carry-over, reducing false positives
• ROX normalization and optimized stabilizers produce a robust, reproducible fluorescent signal
• Room-temperature reaction assembly possible due to antibody hot-start/activation chemistry, improving workflow convenience