Quantabio PerfeCTa qPCR FastMix, UNG ROX 1250R 95077-012

PerfeCTa qPCR FastMix, UNG ROX is a 2X concentrated, ready-to-use qPCR master mix formulated for fast, probe-based real-time PCR workflows. The formulation contains UNG to prevent carry-over contamination and includes ROX reference dye for instruments that require passive reference normalization. The mix is optimized with proprietary buffer and stabilizers for high PCR efficiency and robust fluorescent signal with TaqMan® or TaqMan MGB probe chemistries, enabling rapid cycling and reduced reaction volumes. The product is supplied as 1250 reactions (1250 x 20 µL) in a format equivalent to 10 x 1.25 mL and is identified by part code 95077-012.

Key Features:

• 2X concentrated, ready-to-use reaction cocktail
• Contains Uracil-N-Glycosylase (UNG) to prevent carry-over contamination
• Includes ROX passive reference dye for instruments requiring ROX
• Optimized buffer and stabilizers for TaqMan® and TaqMan MGB probe chemistries
• Pack size: 1250 x 20 µL reactions (10 x 1.25 mL); part code 95077-012

Advantages:

• Enables fast cycling and reduced reaction volumes for increased throughput
• UNG inclusion reduces false positives from carry-over contamination
• ROX normalization improves fluorescence signal consistency on ROX-requiring instruments
• Proprietary buffer/stabilizers deliver high PCR efficiency and robust sensitivity with probe assays