Quantabio PerfeCTa Multiplex qPCR ToughMix, ROX 5000R 95148-05K

PerfeCTa Multiplex qPCR ToughMix, ROX 5000R is a ready-to-use, 5X concentrated multiplex qPCR master mix formulated for probe-based multiplex real-time PCR with a titrated ROX passive reference dye. It provides a single-tube solution that contains AccuStart II Taq DNA Polymerase, optimized reaction buffer and dNTPs for amplification of multiple targets in the same reaction and is intended for molecular biology and qPCR applications requiring multiplex detection and use of ROX normalization.

Key Features:

• Format: Single-tube, 5X concentrated reagent
• Contains AccuStart II Taq DNA Polymerase and optimized reaction buffer with molecular-grade MgCl2 and dATP/dCTP/dGTP/dTTP
• Includes titrated ROX passive reference dye
• Pack size / volume: 1 x 25 mL (configured for 5000 x 25 µL reactions)
• Stabilizers and performance-enhancing additives included

Advantages:

• Optimized for highly multiplexed DNA amplifications and miniaturized reaction volumes
• Formulation designed to tolerate a broad spectrum of PCR inhibitors for robust performance with challenging sample types
• Titrated ROX reference dye enables ROX-normalized instruments to monitor and normalize fluorescence signals
• Demonstrated reagent stability with resistance to repeated freeze–thaw without loss of performance