Quantabio PerfeCTa Multiplex qPCR ToughMix, ROX 250R 95148-250

PerfeCTa Multiplex qPCR ToughMix, ROX is a ready-to-use, concentrated qPCR reaction mix formulated for multiplex real-time PCR assays. Supplied as 250 x 25 µL reactions (1 x 1.25 mL) with ROX reference dye, the ToughMix formulation overcomes many common PCR inhibitors found in environmental, plant, and animal samples and is optimized to support highly multiplexed DNA amplifications in miniaturized reaction volumes. Primary use cases include multiplex probe-based (e.g., TaqMan) real-time PCR applications requiring inhibitor tolerance and a stable, ready-to-use master mix.

Key Features:

• Format: 250 x 25 µL reactions (1 x 1.25 mL)
• Concentration: concentrated, ready-to-use (5X format for multiplex assays)
• Includes: ROX reference dye (formulation with ROX)
• Performance: ToughMix formulation neutralizes a broad spectrum of PCR inhibitors
• Stability/features: reported exceptional reagent stability and anti-foam technology for efficient mixing

Advantages:

• Robust inhibitor tolerance enables reliable amplification from crude or challenging sample types
• Optimized for multiplexing and miniaturized reaction volumes to support multi-target assays
• Ready-to-use concentrated mix (reduces pipetting steps and simplifies reaction setup)
• Incorporated ROX reference dye provides passive reference normalization for compatible instruments
• Enhanced reagent stability and anti-foam formulation support consistent handling and performance