Quantabio PerfeCTa Multiplex qPCR ToughMix, ROX 1000R 95148-01K

PerfeCTa Multiplex qPCR ToughMix is a 2X concentrated, ready-to-use master mix for multiplex real-time PCR that contains engineered hot-start polymerase and optimized buffer components for robust amplification of DNA targets. It is formulated to tolerate common PCR inhibitors found in complex samples and to support multiplex assays (up to four targets) on instruments requiring a ROX passive reference dye. Primary use cases include sensitive multiplex target detection and quantitation from challenging sample matrices in research applications.

Key Features:

• 2X concentrated, ready-to-use master mix
• Compatible with ROX passive reference dye (ROX 1000R)
• Designed for multiplexing up to four targets per reaction
• Engineered hot-start AccuStart polymerase with stabilizers
• Resistant to PCR inhibitors and stable after multiple freeze–thaw cycles (uninhibited after 20× freeze–thaw or 30 days at 22 °C)

Advantages:

• Enhanced inhibitor tolerance enables reliable amplification from crude or challenging sample types
• ROX normalization support ensures accurate fluorescence normalization on compatible real-time PCR instruments
• 2X ready-to-use format reduces pipetting steps and simplifies reaction setup
• Formulation stability and anti-foam technology support reproducible mixing and consistent performance across runs