Quantabio PerfeCTa Multiplex qPCR ToughMix, L-ROX 5000R 95149-05K

PerfeCTa Multiplex qPCR ToughMix, L-ROX is a ready-to-use master mix for multiplex real-time qPCR using hydrolysis probe chemistries. It is formulated for robust amplification of multiple DNA targets in a single reaction and tolerates common PCR inhibitors found in crude or complex samples. Typical use cases include multiplex pathogen detection, high-throughput target quantification, and assays requiring a passive reference dye (Low ROX) for instruments that use ROX normalization.

Key Features:

• Format: Low ROX (L-ROX) passive reference dye
• Pack size / volume: 5000 reactions (25 µL reactions; 1 × 25 mL)
• Concentration: 5X concentrated master mix
• Detection chemistry: optimized for hydrolysis probes (e.g., TaqMan)
• Multiplex capacity: supports simultaneous detection of up to 5 targets
• Enzyme system: contains AccuStart/AccuStart II hot-start DNA polymerase and stabilizers

Advantages:

• Robust performance in presence of common PCR inhibitors, enabling use with crude or challenging sample types
• Enables high-order multiplexing (up to 5 targets) to reduce sample use and increase throughput
• 5X ready-to-use format reduces pipetting steps and simplifies reaction setup
• Includes Low ROX passive reference dye for instruments requiring ROX normalization and an AccuStart hot-start enzyme for high sensitivity and specificity