Quantabio PerfeCTa Multiplex qPCR ToughMix, L-ROX 250R 95149-250

PerfeCTa Multiplex qPCR ToughMix, L-ROX is a concentrated, ready-to-use 5X master mix formulated for multiplex real-time PCR using hydrolysis probe chemistries. It provides a complete reaction buffer including hot-start DNA polymerase, optimized MgCl2 and dNTPs, and L-ROX passive reference dye, enabling sensitive, efficient quantification of multiple DNA targets in a single reaction for applications such as diagnostic real-time PCR, environmental, plant, food, and clinical specimen testing.

Key Features:

• Format: 5X concentrated qPCR master mix
• Dye: L-ROX passive reference dye included
• Pack size: 250 reactions (250 x 25 µL; 1 × 1.25 mL)
• Enzyme: Ultrapure hot-start DNA polymerase (AccuStart-series technology)
• Compatibility: Designed for hydrolysis probe chemistries (e.g., TaqMan®)

Advantages:

• ToughMix formulation neutralizes common PCR inhibitors found in complex sample matrices to support robust amplification
• Concentrated 5X format reduces pipetting steps and conserves reagent volume for multiplex assays
• Included L-ROX reference dye ensures stable normalization for instruments requiring ROX
• AccuStart hot-start enzyme delivers high sensitivity and specificity for multiplex probe-based detection