Quantabio PerfeCTa Multiplex qPCR ToughMix, L-ROX 1000R 95149-01K

PerfeCTa Multiplex qPCR ToughMix, L-ROX (1000R) is a ready-to-use, concentrated master mix formulated for multiplex real-time quantitative PCR using probe-based chemistries. The mix neutralizes a broad spectrum of common PCR inhibitors found in environmental, plant, animal, and clinical specimens and is optimized to deliver high sensitivity, efficiency, and specificity for multi-target assays. This Low-ROX formulation is supplied in a pack size sufficient for 1000 reactions and is intended for research qPCR applications requiring reliable multiplex probe detection on instruments that use low ROX normalization.

Key Features:

• Format: Low-ROX (L-ROX) 2X/5X concentrated multiplex qPCR ToughMix (multiplex-optimized)
• Pack size: 1000 reactions (1000 x 25 µL reaction equivalents; 4 × 1.25 mL aliquots)
• Enzyme system: Ultrapure hot-start AccuStart/AccuStart II DNA polymerase with antibody-mediated hot-start
• Inhibitor tolerance: Engineered ToughMix formulation to neutralize common PCR inhibitors (e.g., heme, humic acid, polysaccharides)
• Includes AccuVue tracer dye and anti-foam technology for improved pipetting and vortex mixing
• Stable storage: stable when stored at −20°C; compatible with fast or standard qPCR cycling protocols

Advantages:

• Enables robust multiplex probe-based detection with high sensitivity and broad linear dynamic range (supports multi-target co-amplification)
• Resilient to inhibitors commonly present in crude or complex sample matrices, improving assay success with challenging inputs
• Stringent antibody hot-start minimizes non-specific amplification and primer-dimer formation for reliable multiplex performance
• AccuVue tracer dye and anti-foam formulation simplify reaction setup and improve pipetting accuracy in plate-based workflows