Quantabio PerfeCTa Multiplex qPCR ToughMix, 1000R 95147-01K

PerfeCTa Multiplex qPCR ToughMix is a 5X concentrated, ready-to-use master mix formulated for multiplex real-time PCR using hydrolysis probe chemistries. It provides a hot-start DNA polymerase and proprietary ToughMix chemistry to enable robust multiplex amplifications in the presence of common PCR inhibitors found in clinical, environmental, plant, animal, and food samples. Typical use cases include probe-based multiplex qPCR assays requiring high sensitivity and reliable performance across multiple targets in a single reaction.

Key Features:

• Concentration: 5X concentrated master mix
• Pack size / reaction count: 1000 x 25 µL reactions (4 × 1.25 mL)
• Enzyme: hot-start DNA polymerase (AccuStart/AccuStart-derived enzyme technology)
• Detection chemistries: compatible with hydrolysis probes (e.g., TaqMan®)
• Storage: recommended storage frozen (−25°C to −15°C); stable conditions noted for handling

Advantages:

• Enhanced tolerance to a broad spectrum of common PCR inhibitors, enabling reliable amplification from challenging sample types
• Optimized for multiplex assays to support simultaneous detection of multiple targets in a single tube
• Ready-to-use 5X formulation reduces pipetting steps and simplifies reaction setup
• Formulation with hot-start enzyme and optimized buffer composition for high sensitivity and specificity in probe-based qPCR