Quantabio AccuStart Long Range SuperMix, 100R 95199-100

AccuStart Long Range SuperMix is a ready-to-use PCR master mix formulated for high-sensitivity amplification of long DNA targets. Supplied as a 100R (100 reaction) pack, the SuperMix contains a blend of two hot-start thermostable DNA polymerases and an optimized buffer to enable efficient amplification of fragments up to 24 kb. It is intended for end-point long-range PCR and downstream applications such as template preparation for Sanger sequencing, NGS library preparation, cloning, and HLA typing, and supports multiplex amplification and a broad range of GC-content targets.

Key Features:

• Pack size: 100R (100 reactions).
• Capable of amplifying long targets up to 24 kb.
• Sensitivity: effective down to 100 pg DNA input.
• Formulation: blend of two hot-start thermostable DNA polymerases with optimized buffer.
• High fidelity/accuracy: reported >10× yield versus Taq; suitable for multiplexing and broad GC-content without separate GC buffer.

Advantages:

• Superior sensitivity enabling amplification from low-template inputs (as little as 100 pg).
• Optimized enzyme blend and buffer support robust long-range amplification up to 24 kb.
• High accuracy and yield compared with standard Taq, improving downstream sequencing and cloning performance.
• Compatibility with multiplexing and broad GC-content targets reduces the need for additional optimization or specialized GC buffers.