Quantabio AccuStart II Taq DNA Polymerase, 1000U 95141-01K

AccuStart II Taq DNA Polymerase is a high-purity, recombinant Taq DNA polymerase with a stringent monoclonal antibody hot-start that keeps the enzyme inactive prior to initial PCR denaturation and is heat-activated for robust PCR performance. The enzyme enables specific and efficient primer extension for conventional PCR applications, supports room-temperature reaction assembly, and is supplied for use in molecular biology workflows requiring reliable DNA amplification.

Key Features:

• Formulation: 1000 U total (5 U/µL, 1 x 200 µL).
• Manufacturer part code: 95141-01K.
• Hot-start mechanism: high-avidity monoclonal antibody that inactivates enzyme until heat activation (1 minute at 94°C).
• Enzymatic activities: 5′→3′ DNA polymerase activity and double-strand specific 5′→3′ exonuclease; no 3′→5′ exonuclease activity.
• Includes 10X PCR Buffer II optimized for higher yields, improved specificity, and enhanced multiplexing capability.

Advantages:

• Enables room-temperature reaction setup by preventing non-specific primer extension prior to thermal activation.
• Provides robust, high-sensitivity PCR amplification with enhanced specificity and yield due to optimized buffer and ultrapure enzyme.
• Supports multiplexing and reliable performance with crude or challenging sample materials owing to optimized buffer formulation.
• Extremely low residual host (E. coli) genomic DNA to minimize background contamination in PCR assays.