Quantabio AccuStart Genotyping ToughMix, ROX 5000R 95116-05K

AccuStart Genotyping ToughMix, ROX is a ready-to-use 2X qPCR master mix formulated for probe-based genotyping and allelic discrimination assays (SNP detection). It is optimized to provide sensitive, precise detection of target alleles and improved cluster separation for single-nucleotide polymorphism assays, and is suitable for use with reactions that require a ROX passive reference dye. The formulation neutralizes a broad spectrum of PCR inhibitors to support amplification directly from challenging sample types such as crude extracts, dried blood spot punches, plant tissue, environmental sources, and clinical specimens.

Key Features:

• Formulation: 2X concentrated ready-to-use master mix
• Includes: ROX passive reference dye (high ROX/ROX normalization for compatible instruments)
• Performance enzyme: Ultrapure AccuStart II hot-start Taq DNA polymerase
• Application: Optimized buffer chemistry for probe-based genotyping and SNP allelic discrimination
• Inhibitor tolerance: ToughMix technology neutralizes common PCR inhibitors from complex sample matrices
• Format/Size indicator: 5000R
• Manufacturer part code: 95116-05K

Advantages:

• Enhanced allelic discrimination and improved cluster separation for SNP genotyping assays
• Robust amplification from crude or inhibitor-containing samples due to ToughMix inhibitor-neutralizing chemistry
• High sensitivity and precision driven by ultrapure AccuStart II hot-start enzyme
• Simplified reaction setup using a 2X ready-to-use mix with ROX reference dye and plate-loading AccuVue dye