Bio-Techne - RnD Systems, MagCellect CD24- CD44+ Breast Cancer Stem Cell Isolation Kit (1 Kit), MAGH111

The MagCellect CD24- CD44+ Breast Cancer Stem Cell Isolation Kit is a complete magnetic cell separation kit designed to enrich CD24low/– CD44+ human breast cancer stem cells using a two-step negative then positive selection workflow. The kit isolates CD24low/– cells by removing CD24+ cells and subsequently selects CD44+ cells with biotinylated antibodies and streptavidin-conjugated magnetic beads, and includes fluorochrome-conjugated detection antibodies for flow cytometric assessment of recovered cells.

Key Features:

• Format: 1 Kit (Catalog code MAGH111)
• Species: Human
• Components include: APC-conjugated Anti-Human CD24 Detection Antibody (0.2 mL, 20 tests) and PE-conjugated Anti-Human CD44 Detection Antibody (0.2 mL, 20 tests)
• Biotinylated antibodies: Anti-Human CD24 (0.5 mL, 20 tests) and Anti-Human CD44 (0.2 mL, 20 tests)
• MagCellect Streptavidin Ferrofluid: 3 mL; 10X MagCellect Plus Buffer: 50 mL
• Storage: Store unopened product at 2 °C to 8 °C; reagents stable for 12 months. DO NOT FREEZE

Advantages:

• Enriches for CD24low/– CD44+ breast cancer stem cells that have been shown to form tumors in mice, enabling downstream functional or analytical studies
• Two-step negative then positive magnetic selection increases purity by removing unwanted CD24+ cells before selecting CD44+ cells
• Includes fluorochrome-conjugated detection antibodies for immediate flow cytometric assessment of isolation efficiency